Example sentences of "[prep] a [unc] agarose " in BNC.
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| 1 | The amplified products were electrophoresed through a 1.5% agarose gel stained with ethidium bromide and viewed under ultraviolet light . |
| 2 | Probes were prepared by random oligonucleotide labelling with digoxigenin of the products of the polymerase chain reaction electrophoresed through a 1.5% agarose gel , excised , and purified with Gene-Clean ( Bio 101 , La Jolla , California ) . |
| 3 | For Southern blots genomic DNA was digested with Eco RI , electrophoresed through a 0.8% agarose gel , transferred with 20xSSC to Hybond N+ ( Amersham ) and fixed in 0.4% NaOH . |
| 4 | Eco RI-digested DNA in the size range 2.2–2.7kb ( S. macroura ) and 4.0–4.4kb ( M. eugenii ) was recovered from a 0.8% agarose gel using Geneclean ( Bio 101 , Inc. ) , ligated into lambda gt10 ( BRL ) and packaged with the Lambda In Vitro packaging kit ( Amersham ) following the manufacturers ' instructions . |
| 5 | The samples were subjected to electrophoresis in a 0.8% agarose gel in TBE buffer for 16 hr at 1.0 V/cm at 20°C . |
| 6 | The PCR amplified samples were analyzed on a 1.2% agarose gel . |
| 7 | The samples analyzed on a 1.2% agarose gel , the fragments purified and sequenced . |
| 8 | PCR was performed as described earlier , and the samples analyzed on a 1.2% agarose gel . |
| 9 | Right S-KN-SH : SKNSH DNA was restricted with SstI run on a 1.5% agarose gel . |
| 10 | The samples were resolved on a 0.7% agarose gel migrated at 4°C at 40 volts . |
| 11 | The samples were resolved on a 0.7% agarose gel . |
| 12 | The reactions were stopped by EDTA/SDS as described above and the DNA extracted and resolved on a 1.3% agarose gel . |
| 13 | An aliquot of each reaction ( 5 µl ) was resolved on a 1.3% agarose gel and the position of the cDNA visualized by autoradiography . |
| 14 | At the end of the incubation , SDS ( 1% ) was added , NCp7 removed by phenol extraction and the nucleic acids resolved on a 1.3% agarose gel . |
| 15 | Five µl aliquots were resolved on a 1.3% agarose gel . |
| 16 | DNA samples were electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and probed with a mixture of 32 P-labeled pBR322 DNA ( 2.5 ng/ml ; 10 9 cpm/µg ) and 32 P-labeled λ DNA ( 0.5 ng/ml ; 10 8 cpm/µg ) . |
| 17 | Genomic DNA ( 10 µg ) from the ura5-6 strain was digested with different restriction enzymes , electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and probed with 32 P-labeled ( T 2 AG 3 ) 4 . |
| 18 | Genomic DNA from the ura5-6 strain was treated with Bal 31 nuclease for 0 ( lane 1 ) , 0.5 ( lane 2 ) , 2 ( lane 3 ) , 7.5 ( lane 4 ) , 15 ( lane 5 ) , or 60 min ( lane 6 ) and subsequently digested with Sal I. ( A ) DNA samples ( 10 µg ) were electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and hybridized with 32 P-labeled ( T 2 AG 3 ) 4 . |
| 19 | Total DNA ( 1 µg ) was electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and hybridized with a mixture of 32 P-labeled pBR322 DNA ( 2.5 ng/ml ; 10 9 cpm/µg ) and 32 P-labeled λ DNA ( 0.5 ng/ml ; 10 8 cpm/µg ) . |
| 20 | Total DNA ( 1 µg ) was digested with Sal I , electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and probed with a mixture of the 32 P-labeled 1.55 kbp Eco RI fragment carrying the ura5 gene ( 2.5 ng/ml ; 10 9 cpm/µg ) and 32 P-labeled λ DNA ( 0.5 ng/ml ; 10 8 cpm/µg ) . |