Example sentences of "[n mass] [adv] shown " in BNC.
Next page| No | Sentence |
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| 1 | Untranslated sequences upstream of the coding regions 1 and 2 were found to be unrelated , and attempts to identify transcripts that contain linked 1 and 2 sequences by library screening or through RT-PCR experiments were unsuccessful ( data not shown ) . |
| 2 | As the same alanine and glycine codons are present at these positions in both human and bovine cDNA sequences and because the human gene , where analysed ( data not shown ) , has the same intron/exon boundaries , we suggest that these conclusions are applicable to the GGF gene structure and to the mRNA splicing patterns in both species . |
| 3 | Similar results to GGFHFB1 were obtained with the bovine cDNA clone GGFBPP5 ( data not shown ) . |
| 4 | Puromycin-resistant cells that expressed Bcl-2 protein ( Rat-1/c-Myc-ER/Bcl-2 cells ) were identified by immunoblotting ( Fig. 1 a ) and immunocytochemistry ( data not shown ) using anti-Bcl-2 monoclonal antibodies . |
| 5 | In neither case did Bcl-2 expression overcome the cytostatic effect of the drugs ( data not shown ) . |
| 6 | Accordingly , all Max proteins tested ( Fig. 1 ) bind DNA as homo-oligomers in yeast extracts ( ref. 9 , and data not shown ) , as recently reported for bacterially expressed Max . |
| 7 | For example , Max C and Max85 proteins seem to allow better Myc+Max function than full-length Max ( Fig. 2 a ) , although all Max proteins were expressed at similar levels ( data not shown ) . |
| 8 | Consistent with this idea , Myc mutants with deletions of either helix-loop-helix or leucine zipper domains did not show any activity , though positive controls are not available for these mutants ( data not shown ) . |
| 9 | The Myc transactivation domain also functions in yeast when fused to heterologous DNA-binding domains ( LexA-Myc ( ref. 22 ) and 1–235Myc-SRF , data not shown ) . |
| 10 | Additional experiments with proteins initiated at their own start codons indicated that the extensions do not significantly alter the behaviour of Myc , Max1 , Max2 , Max1 C and Max2 C ( data not shown ) . |
| 11 | The relatively higher efficiencies of Myc with Max2 , Max2 C or Max85 , compared with Max1 or Max1 C , may in part be due to differences in affinity for DNA ( ref. 9 , and data not shown ) . |
| 12 | Activity is dependent on the presence of reducing agent and shows no requirement for divalent cations ( data not shown ) . |
| 13 | In the presence of 100mM K + and 10mMNa + ions at 38°C , only the six aromatic resonances with identical chemical shift to those of the low-temperature form in 100mMNa + alone were observed ( data not shown ) . |
| 14 | Instead we used through-bond couplings ( data not shown ) to assign proton resonances within each deoxyribose , and 1 H- 31 P couplings ( Fig.2 a ) to connect them . |
| 15 | Therefore the assignment scheme shown in Fig.3 a was tested by observing the spectrum of the hexadeoxynucleotide analogue d ( UG 4 T ) ( data not shown ) . |
| 16 | We observe non-hydrogen-bonded amino protons at 5.6–5.8p.p.m. , with NOE connectivities to the imino and the hydrogen-bonded amino protons ( data not shown ) . |
| 17 | In addition , four strong and two weak imino resonances ( data not shown ) disappear together with the broad set of aromatic resonances at high temperature ( 70°C ) . |
| 18 | A sequential connectivity pathway is observed between aromatic and H1' protons , and between aromatic and H2' , H2 protons ( data not shown ) as expected for right-handed helical DNA . |
| 19 | One monoclonal antibody , KS128 , demonstrated high expression on freshly prepared peripheral blood lymphocytes ( PBL ) , monocytes and granulocytes ( data not shown ) and was selected for further study . |
| 20 | Antibody KS128 did not inhibit ICAM-3/LFA-1 adhesion ( data not shown ) . |
| 21 | As in wild-type littermates , these cells are small and negative for IL-2R ( data not shown ) . |
| 22 | As in wild-type littermates this thymocyte population includes two subpopulations of similar cell numbers : immature , IL-2R-positive , TCR-negative cells , and mature , IL-2R-negative , γ TCR-positive cells ( data not shown ) . |
| 23 | Finally , the TCR- β mutant thymus contains a few CD4 + SP and CD8 + SP cells , of which between half and two-thirds are γ T cells ( data not shown ) . |
| 24 | Cloning and sequencing experiments confirmed that these transcripts are indeed derived from productively or non-productivity rearranged TCR -α genes ( data not shown ) . |
| 25 | The TCR -β and CD4-positive cells were not stained by two different anti-TCR C antibodies ( 3A10 and GL3 ) and are therefore unlikely to bear mixed TCR β heterodimers ( data not shown ) . |
| 26 | We observed an equally small population of CD4-positive dull TCR- β -positive cells in the periphery of TCR- β transgenic TCR α mutant mice ( data not shown ) . |
| 27 | But TCR- -positive DP cells have been detected in the late embryonic thymus and about 1% of TCR - -positive cells in the postnatal TCR- β mutant thymus are DP ( data not shown ) . |
| 28 | We have also obtained TCR- β and TCR - mutant mice in a ( 129/OlaxC57BL/6 ) background and have found no effect of genetic background on thymocyte numbers and percentage of DP cells ( data not shown ) . |
| 29 | The DN TCR-negative thymocytes in the TCR- β mutant mice are predominantly IL-2 receptor- and Sca-1 positive , and class I MHC high ( data not shown ) . |
| 30 | Partially purified splenic dendritic cells and preparations enriched in lymph node dendritic cells did not stain with ECCD-2 or PCD-1 ( data not shown ) . |