Example sentences of "cell were [verb] with [art] " in BNC.
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| 1 | Suspensions of HGT-1 cells were preincubated with the activator of protein kinase C , 12- O -tetradecanoylphorbol 13-acetate ( TPA , 100 nmol/l ) , for 10 minutes . |
| 2 | Cells were treated with a range of chemical and physical insults before analysis of mRNA ( Fig. 1 b ) and the pattern of induction of CL100 mRNA was compared with that for haem oxygenase , a gene transcriptionally induced in human cells by oxidant stress . |
| 3 | The cells were counted with a modified Neubauer haemocytometer . |
| 4 | After three to four days incubation at 37°C the cells were harvested with 0.025% trypsin/0.5% EDTA ( Sigma ) and viable cells were counted with a haemocytometer ( viability was assessed by trypan blue exclusion ) . |
| 5 | HT-29 cells were incubated with the mannosidase I inhibitor DMJ for various times and Β 1 integrins were immunoprecipitated from I-surface labelled cells and analysed by SDS-PAGE followed by autoradiography . |
| 6 | Results were comparable when target cells were infected with a recombinant vaccinia virus making the influenza M1 matrix protein ( M1-vac ) and the M1-specific CTL line 4–30 was used ( Table 2b ) . |
| 7 | When Spodoptera frugiperda cells were infected with the recombinant virus ( AcBT-3/2/7/5 ) expressing the four major structural genes of BTV , double-capsid , virus-like particles consisting of VP2 , VP3 , VP5 and VP7 of BTV were assembled . |
| 8 | To assess the ability of the quadruple gene expression vector to make virus-like particles , subconfluent monolayers of S.frugiperda insect cells were infected with the recombinant AcBT-2/3/7/5 virus , harvested 48 h post-infection , lysed with the nonionic detergent Nonident P-40 , and released particles were purified on discontinuous sucrose gradients as described in Methods . |
| 9 | Cells were transfected with the vector pMT2 without insert ( ○ ) , with pMT2-PLC- β1 ( fl ) or pMT2-PLC- β2 ( i ) . |
| 10 | However , when cells were transfected with the longer N-Oct 3 cDNA ( Figure 3a , lane 6 , Fig. 3b plasmid IV ) there was an inverse intensity of the two N-Oct 5 bands . |
| 11 | Control cells were transfected with the same amount of Bluescribe DNA . |