Example sentences of "use as substrate " in BNC.

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1 Ψ40 was formed at relatively low levels when the wt-like tRNA Asp ( mutant a ) was used as substrate ( Fig. 2C and Table I ) .
2 After washing , 100 µl optimal dilution of rabbit anti-goat IgG conjugated with alkaline phosphatase was added and 100 µl p-nitrophenyl-phosphate ( 1 mg/ml ) was used as substrate .
3 Rabbit anti-tissue type plasminogen activator was used as catching antibody , an anti-tissue type plasminogen activator horseradish peroxidase conjugate ( Biopool , Sweden ) as second antibody and 3,3',5,5' tetramethylbenzidine was used as substrate .
4 Diaminobenzidine was used as substrate in the techniques .
5 Monoclonal antibodies were variously employed at dilutions of 1/15–1/50 in a two step indirect peroxidase method , where DAB was used as substrate and NiCl as enhancing agent : nuclei were stained with Mayer 's haematoxylin .
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