Example sentences of "use as substrate " in BNC.
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1 | Ψ40 was formed at relatively low levels when the wt-like tRNA Asp ( mutant a ) was used as substrate ( Fig. 2C and Table I ) . |
2 | After washing , 100 µl optimal dilution of rabbit anti-goat IgG conjugated with alkaline phosphatase was added and 100 µl p-nitrophenyl-phosphate ( 1 mg/ml ) was used as substrate . |
3 | Rabbit anti-tissue type plasminogen activator was used as catching antibody , an anti-tissue type plasminogen activator horseradish peroxidase conjugate ( Biopool , Sweden ) as second antibody and 3,3',5,5' tetramethylbenzidine was used as substrate . |
4 | Diaminobenzidine was used as substrate in the techniques . |
5 | Monoclonal antibodies were variously employed at dilutions of 1/15–1/50 in a two step indirect peroxidase method , where DAB was used as substrate and NiCl as enhancing agent : nuclei were stained with Mayer 's haematoxylin . |