Example sentences of "from plasmid [no cls] " in BNC.
Next page| No | Sentence |
|---|---|
| 1 | The DNA templates used were either supercoiled pFRC64 plasmid ( 2 nM ) or a 340 bp PvuI-BamHI fragment ( 2 nM ) , obtained from plasmid pFRC64 , which gives rise to run-off transcripts 143 nt long from P A2b and 79 nt long from P A3 . |
| 2 | Ptyr 2 DNA was prepared from plasmid pMLB1045 as previously described [ 24 ] and labelled at the 3'-end of the Eco R1 site . |
| 3 | The 160 base pair tyr T DNA fragment prepared from plasmid pKMĪ-98 as previously described and labelled at the 3'-end of the Eco R1 site [ 3 ] . |
| 4 | The gene 62 fragment used in the construction of the plasmid expressing VT5 was isolated from plasmid pV18 ( 39 ) which is a derivative of p140 ( 28 ) with a 12bp EcoRI linker oligonucleotide inserted at codon 472 . |
| 5 | The black box represents the NcoI-EcoRI oligonucleotide adaptor inserted to maintain the 140k reading frame and to facilitate the insertion of the EcoRI-BstEII gene 62 fragment from plasmid pV17 into the vector p585T7a . |
| 6 | The remaining gene 62 fragments were all isolated from plasmid pV17 ( refer to materials and methods ) . |