Example sentences of "at [num] g " in BNC.
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1 | Supernatants were combined and centrifuged at 16500 g for 25 minutes to obtain a lysosome enriched pellet . |
2 | Latency was calculated as follows : Increased lysosomal fragility is indicated by a decrease in latency. ( ii ) Determination of the proportion of total enzyme released into the supernatant after resedimentation of lysosomes from the incubated samples by centrifugation at 16500 g fr 25 minutes . |
3 | Cells were scraped into extraction buffer , incubated for 1h at 4°C with gentle vortexing every 10 min , and then centrifuged at 18,000 g for 20min . |
4 | The homogenates were centrifuged at 3000 g for 15 minuts . |
5 | For this the samples were thawed and centrifuged at 3000 g for 10 minutes to remove the mucus . |
6 | To minimise loss of APGPR immunoreactivity on storage , each urine sample was centrifuged at 3000 g for 20 minutes , the supernatant suspended in a boiling bath for 10 minutes , centrifuged for five minutes at 10000 g , and the supernatant aliquoted and stored at -20°C until assayed . |
7 | Each blood sample was immediately made 20 mM with zinc acetate , allowed to clot , and within 2.5 hours of collection , centrifuged at 3000 g for 20 minutes , the separated serum mixed with an equal volume of ELISA immunoassay buffer containing 50 mM TRIS/HCL , 0.05% ( wt/vol ) casein , 3.1 mM NaN 3 , 10 mM ethylenediaminetetra-acetic acid , 0.05% ( wt/vol ) Tween 20 at pH 7.2–7.4 , and suspended in a boiling bath for 10 minutes . |
8 | ( v ) Adhere the cells on to their glass coverslips by centrifuging them at 500 g for 10 min at 20-C in a HB-4 rotor of a Sorvall RC-5 centrifuge . |
9 | After centrifugation for 60 minutes at 96000 g , cell membranes were collected as a white interfacial band and pelleted by centrifugation at 48000 g for 30 minutes . |
10 | Cellular and nuclear debris were removed from the lysate by a 5-min centrifugation at 300 g and membranes pelleted by a 30-min centrifugation at 100,000 g . |
11 | The resultant supernatant was removed immediately and respun at 300 g to yield a cell free plasma . |
12 | The supernatant was removed immediately and respun at 300 g for 10 minutes to yield cell free plasma . |
13 | The drivative was dried and extracted with 3×200 µl hexane and centrifuged in a microtube at 13400 g for 10 minutes . |
14 | The expressed virus-like particles ( VLPs ) were purified by lysing infected cells as described above followed by centrifugation on a discontinuous sucrose gradient 30%:50% [ wt/vol ] in 0.2 M Tris hydrochloride [ pH 8.0 ] at 85,000 g for 3 h . |
15 | Blook ( 5 ml ) was layered above an 8 ml bilayer of hypaque 1017 and 1119 in equal volumes and centrifuged at 700 g for 25 minutes . |
16 | The supernatant was removed and centrifuged at 100 g for five minutes . |
17 | Five millilitres of cell free plasma were added to the cell suspension and centrifuged at 100 g for five minutes . |
18 | The supernatant was removed and centrifuged at 100 g for 5 minutes . |
19 | Five ml of cell free plasma were then added to the cell suspension and centrifuged at 100 g for 5 minutes . |
20 | After centrifugation for 60 minutes at 96000 g , cell membranes were collected as a white interfacial band and pelleted by centrifugation at 48000 g for 30 minutes . |
21 | After sonication , the sample was centrifuged at 150000 g for one hour to yield a faintly opalescent supernatant consisting of a homogeneous population of cholesteryl oleate vesicles . |
22 | The samples were immediately ultracentrifuged at 100000 g for two hours at 37°C ( Hitachi 55P-72 , Tokyo , Japan ) . |
23 | Homogenates were centrigued at 40000 g for 90 minutes at 4°C , and the pellet was resuspended in homogenisation buffer . |
24 | After reflux the sample was cooled on ice and centrifuged at 1500 g for 15 minutes . |
25 | Each tube was centrifuged at 1500 g for 10 minutes and after separation , the radioactivity of the pellet was counted in an LKB 1271 RIAGAMA counter ( Turku , Finland ) . |
26 | Twenty millilitres were centrifuged at 1500 g for 10 minutes to obtain cell free plasma . |
27 | This suspension was centrifuged at 1500 g for 10 minutes . |
28 | Ileal contents were incubated in a shaking waterbath ( 5 minutes at 37°C ) and subsequently centrifuged for 10 minutes at 15000 g ( Eppendorf 5415 ) . |
29 | After homogenisation , the samples were incubated in a shaking waterbath ( 1 hour at 37°C ) , followed by centrifugation for 10 minutes at 15000 g . |
30 | Cultures were pelleted by centrifugation at 17000 g for 15 minutes , coded in Houston then sent to London to be assayed . |