Example sentences of "legend [prep] [noun sg] [adj] " in BNC.
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| 1 | To demonstrate the specificity of the IS902 polymerase chain reaction used for M avium subsp silvaticum , genomic DNA from 25 bacterial species including 21 mycobacteria ( see legend for Figure 1 ) was subjected to IS902 polymerase chain reaction in accordance with the protocol described below . |
| 2 | For abbreviations and references see legend of Fig. 2 . |
| 3 | To date , five species are known which contain a group I intron at an identical position of the mtLSUrRNA ( Fig. 3 ) : the four ascomycetes P.anserina ( intron ri2 ) , N.crassa ( intron ri1 ) , A.nidulans ( intron ri1 ) , S.cerevisiae ( intron ri1 ) and P.wickerhamii ( intron ri1 ) ( references are given in the legend of Fig. 3 ) . |
| 4 | Abbreviations see legend of Fig. 3 . |
| 5 | The Mam I- Sfa NI , Mam I- Sau 3A and Xba I- Pvu II deoP2 fragments were purified by electrophoresis on polyacrylamide gels after labelling of the 5'-end of the Mam I site with polynucleotide kinase in the presence of γ- 32 P-ATP and the 3'-end of the Xba I site with Klenow fragment in the presence of α- 32 P-dCTP. 0.8 nM of the labelled fragments and various concentrations ( indicated in the Legend in Fig. 3 ) of CytR or CRP were incubated in binding buffer ( 10 mM Tris HCl ( pH 7.8 ) , 50 mM KCl , 1 mM EDTA , 50 µg/ml acetylated bovine serum albumine , 1 mM dithiothreitol , 0.05 % Nonidet-P40 ) containing 20 µg/ml competitor DNA ( pGEM4 obtained from Promega ) for 30 min. at 37°C in a total volume of 10 µl ( 6 ) . |
| 6 | Details of wounding are given in the legend to Table 1 . |
| 7 | Sugar conformations and glycosidic rotation angles have been estimated as described in the legend to Table 1 . |
| 8 | Trunk fragments , with attached -AER and +AER limbs , were transferred to tubes ( Falcon no. 2057 ; 4–6 trunk fragments per tube ) containing 1-ml aliquots of equilibrated serum-free defined culture medium ( modified Biggers medium ) , growth factors added ( see legend to Table 1 ) , and the tubes rotated ( 30r.p.m. at –75° angle ) in a 5% CO 2 incubator at 37°C . |
| 9 | The region shown includes the helix that contains met-31 , which was replaced by seleno-methionine ( see text and legend to Table 1 ) to obtain phases by anomalous dispersion . |
| 10 | Details of crystallization , data collection , MIR phase determination , structure refinement and overall structure description are given in Table 1 and the legend to Fig. 1 . |
| 11 | In the culture system used ( see legend to Fig. 1 ) , trunk fragments are isolated containing either forelimb or hindlimb buds , and the AER is removed from one limb ( -AER limb ) to expose the underlying mesenchyme , but kept intact on the other ( +AER limb ) . |
| 12 | Trunk fragments with attached limbs ( +AER and -AER ) were cultured as described in the legend to Fig. 1 for 48h in serum-free medium supplemented as stated ( resupplemented after 24h ) . |
| 13 | A suitable plasmid for expressing CFTR protein was constructed in the vector pREP8 ( see legend to Fig. 1 ) . |
| 14 | The structure of the complex ( Fig. 1 ) shows two repressor dimers bound to a single duplex oligonucleotide , with the noncrystallographic 2-fold axes of the dimers roughly coincident with local DNA 2-fold axes passing through the centres of met box sequences , between base pairs 4 and 5 , and 12 and 13 , respectively ( see legend to Fig. 4 for DNA numbering scheme ) . |
| 15 | His responses were at chance level ( see legend to Fig. 4 ) . |
| 16 | 32 P-labelled fragments ( final concentration 1 nM ) , proteins at the concentrations stated in the legend to Fig. 4 and competitor DNA were incubated as in the gel retardation experiments . |
| 17 | b , Relative β -galactosidase units ( as defined in legend to Fig. 2 ) in S62L cells expressing the indicated proteins . |
| 18 | The results supported the figural prediction ( see legend to Fig. 2 ) , demonstrating that the critical variable was the location of the dividing contour in relation to the green figure , rather than in relation to the patient or to the display as a whole . |
| 19 | Peptide stimulations and CTL assays were done essentially as described in the legend to Fig. 2 . |
| 20 | This conformation is rigidly maintained by three hydrogen bonds ( see legend to Fig. 2 a ) . |
| 21 | Further analysis of the deletions using additional sections of the 5' half of the cDNA clone 14–6 ( see legend to Fig. 2 c for details ) confirmed that patients D and E both contain normal restriction fragments derived from the 5' end of the gene in addition to those detected near the 3' end with probe 7C11 . |
| 22 | See legend to Fig. 2 for details of methods and hybridization probes . |
| 23 | Introduction of plasmid DNA into the mouse airways was as described in the legend to Fig. 2 . |
| 24 | He showed the usual effect of symmetry on figure-ground segregation , tending to report the colour of the symmetrical shapes , with no bias towards reporting the rightmost colour ( see legend to Fig. 3 ) . |
| 25 | The observed increase in -AER limb length results from increased cell division in distal limb mesenchyme , as demonstrated by the finding that the frequency of bromodeoxyuridine(BrdU)-labelled mesenchyme cells is 33% ( 4.5% ) in FGF-4 stimulated -AER limbs , but only 4% ( 0.7% ) in contralateral +AER limbs ( method described in the legend to Fig. 3 ) . |
| 26 | The alignment with structurally equivalent residues of CAP ( defined in legend to Fig. 3 ) is also shown . |
| 27 | Experimental conditions were as described in the legend to Figure 5 . |