Example sentences of "centrifuge at [num] [noun] " in BNC.
Next page| No | Sentence |
|---|---|
| 1 | Centrifuge at 12,000 rpm for 10 minutes . |
| 2 | ( v ) Resuspend the pellet in 5 m1 of 45% glacial acetic acid , then immediately centrifuge at 150 g for 5 min . |
| 3 | Draw off the cell suspension and centrifuge at 150 g for 10 min in a conical centrifuge tube . |
| 4 | Centrifuge at 50 g for 5 min with slow acceleration . |
| 5 | The free and protein bound leucine in the tissue biopsies were separated by pulverising the frozen tissue , homogenising in 0.2 M HClO 4 at 4°C and centrifuging at 2800 g for 20 minutes . |
| 6 | Aspirates were immediately treated with anticoagulant ( 9:1 v/v 10% EDTA ) and centrifuges at 3500 rpm at 4°C for 10 minutes . |
| 7 | For this the samples were thawed and centrifuged at 3000 g for 10 minutes to remove the mucus . |
| 8 | Each blood sample was immediately made 20 mM with zinc acetate , allowed to clot , and within 2.5 hours of collection , centrifuged at 3000 g for 20 minutes , the separated serum mixed with an equal volume of ELISA immunoassay buffer containing 50 mM TRIS/HCL , 0.05% ( wt/vol ) casein , 3.1 mM NaN 3 , 10 mM ethylenediaminetetra-acetic acid , 0.05% ( wt/vol ) Tween 20 at pH 7.2–7.4 , and suspended in a boiling bath for 10 minutes . |
| 9 | The supernatant was removed and centrifuged at 100 g for five minutes . |
| 10 | Five millilitres of cell free plasma were added to the cell suspension and centrifuged at 100 g for five minutes . |
| 11 | The supernatant was removed and centrifuged at 100 g for 5 minutes . |
| 12 | Five ml of cell free plasma were then added to the cell suspension and centrifuged at 100 g for 5 minutes . |
| 13 | After reflux the sample was cooled on ice and centrifuged at 1500 g for 15 minutes . |
| 14 | After removal of the stomach , a 50–100 mg full thickness strip from the oxyntic region was immediately homogenised in 3.5 ml of 0.01 M hydrochloric acid and centrifuged at 2000 g for 15 minutes before removal of the supernatant for the following measurements : |
| 15 | Cells were scraped into extraction buffer , incubated for 1h at 4°C with gentle vortexing every 10 min , and then centrifuged at 18,000 g for 20min . |
| 16 | Supernatants were combined and centrifuged at 16500 g for 25 minutes to obtain a lysosome enriched pellet . |
| 17 | Leucocytes were washed twice in 10 ml solution ( 37°C ) containing 100 nM/l indomethacin ( KHI ) to inhibit prostglandin synthesis , centrifuged at 200 g for 10 minutes , and resuspended in 5 ml KHI . |
| 18 | One portion of the homogenates was boiled for 10 minutes , centrifuged at 5000 g for 10 minutes and the supernatant stored at -20°C for the later determination of histamine . |
| 19 | The suspension was then layered onto 5 ml of buffer C ( equivalent to buffer B , but containing 30% sucrose ) and centrifuged at 2500 rpm at 4°C for 30 min . |
| 20 | This was placed in a sterile universal tube and centrifuged at 14000 revolutions per minute for 4 minutes as previously described to elute sample DNA . |
| 21 | Blook ( 5 ml ) was layered above an 8 ml bilayer of hypaque 1017 and 1119 in equal volumes and centrifuged at 700 g for 25 minutes . |
| 22 | Briefly , tumours or mucosal scrapings were homogenised in TEA buffer ( 250 mM sucrose , 10 mM triethanolamine ( pH 7.6 ) containing 1 mM phenylmethylsuphonylfluoride ( PMSF ) and 1 mM N-ethylmaleimide as protease inhibitors ) on ice and centrifuged at 1000 g for 10 minutes . |
| 23 | Cell ( 10 6 ) were added to 5ml Falcon polystyrene tubes ( Becton Dickinson , Lincoln Park , NJ , USA ) , and centrifuged at 1000 g for 5 minutes . |
| 24 | Cells were harvested by incubation with 1 mM EDTA in tris buffered saline for 20 minutes and centrifuged at 1000 rpm for 10 minutes . |
| 25 | pHIVCG9.9 HIV-1 plasmid DNA was incubated with NCp7 or NCp7 mutants in a final volume of 20 µl as described above and the reactions subsequently centrifuged at 12000 rpm for 30 min at 4°C . |
| 26 | Serum samples were stored at -80°C without preservatives and centrifuged at 105000 g for 90 minutes before use . |
| 27 | Cell suspensions were centrifuged at 200 g for 10 minutes and the pellet of cells added to the organ bath the bathing fluid of which was used to resuspend them for transfer . |
| 28 | The homogenates were centrifuged at 3000 g for 15 minuts . |
| 29 | To minimise loss of APGPR immunoreactivity on storage , each urine sample was centrifuged at 3000 g for 20 minutes , the supernatant suspended in a boiling bath for 10 minutes , centrifuged for five minutes at 10000 g , and the supernatant aliquoted and stored at -20°C until assayed . |
| 30 | The clotted sample was centrifuged at 3000 rpm for 10 minutes and the serum separated . |