Example sentences of "in [adj] µl [noun] " in BNC.
Next page| No | Sentence |
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| 1 | The final volume of the template depends on the volume of the PCR reaction e.g. PCR products from a 30 µl reaction are dissolved in 10 µl H 2 O and PCR products from a 50 µl reaction are dissolved in 30-50 µl H 2 O. |
| 2 | Rabbit platelets were washed as described previously : aspirinated platelets ( 1.6×10 8 ) in 300 µl Tyrode 's solution , containing 2.5% gelatin and the ADP scavenger mixture creatine phosphate ( 1 mM ) and creatine phospholinase ( 10 IU/ml ) were stirred in an aggregometer ( Icare , Marseille , France ) . |
| 3 | The extract was dried under a stream of nitrogen , redissolved in 30 µl cloroform and spotted onto gel plates coated with silica ( Whatman HP-KF 10×10 cm ) . |
| 4 | The pellet was dissolved in 100 µl DEPC 0.8% water and treated with 200 U HPLC grade DNAse 1 ( Pharmacia ) in buffer Tris 50 mM ( pH 8 ) , MgCl 2 10mM , NaCl 50 mM with 200 U/ml RNAsine at 37°C , 30 min , then extracted with phenol-chloroform . |
| 5 | 1 µg of mRNA was resuspended in 10 µl dd H 2 O containing 2 µg oligo dt ( 11–12 mer , Promega Biotec , Madison , WI ) and was incubated at 65°C for 10 minutes . |
| 6 | The final volume of the template depends on the volume of the PCR reaction e.g. PCR products from a 30 µl reaction are dissolved in 10 µl H 2 O and PCR products from a 50 µl reaction are dissolved in 30-50 µl H 2 O. |
| 7 | The expelled 1 4 CO 2 was trapped in 50 µl Protosol ( New England Nuclear ) and counted after adding scintillation fluid . |
| 8 | 3 µg double stranded pMc5–8 DNA ( 20 ) , prepared from transformed RP4182 and therefore C 5 -unmethylated , was incubated at 37°C for 30 min with 1 µg purified Dcm or Dcm/Cys177Ser protein in 50 µl DCM reaction buffer containing 80 µM SAM . |
| 9 | 12 µg C 5 -unmethylated , double stranded pMc5–8 DNA ( 20 ) ( corresponding to 5 pmol phasmid DNA or 80 picoequivalents of DNA cytosine residues susceptible to methyltransfer by Dcm ) were mixed with 50 ng Dcm ( 1 pmol ) or 500 ng Dcm/Cys177Ser ( 10 pmol ) and 150 pmol [ 3 H ] -SAM ( 10 µCi , 73 Ci/mmol ) in 110 µl DCM reaction buffer ( total volume ) . |
| 10 | R values were measured in 40 µl droplets of each solution on a glass coverslip . |
| 11 | The supernatant was transferred to a glass gas liquid chromatography vial ( Chromacol Ltd , London , UK ) dried and resuspended in 200 µl hexane . |
| 12 | Plasma gastrin concentration was determined in 200 µl plasma using a double antibody liquid phase 1 2 5 I-radioimmunoassay ( Diagnostic Products Corporation , Los Angeles , Calif , USA ) . |
| 13 | Platelets were stirred in 400 µl buffer containing 2.6 mM KCl , 1 mM MgCl 2 , 137 mM NaCl , 12 mM NaHCO 3 , 1.3 mM CaCl 2 , 5.5 mM glucose , 0.25 gelatine , 1 mM creatine phosphate and 10 IU/ml creatine phosphokinase ( pH 7.4 ) . |