Example sentences of "20 [unc] [noun sg] " in BNC.
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| 1 | The Mam I- Sfa NI , Mam I- Sau 3A and Xba I- Pvu II deoP2 fragments were purified by electrophoresis on polyacrylamide gels after labelling of the 5'-end of the Mam I site with polynucleotide kinase in the presence of γ- 32 P-ATP and the 3'-end of the Xba I site with Klenow fragment in the presence of α- 32 P-dCTP. 0.8 nM of the labelled fragments and various concentrations ( indicated in the Legend in Fig. 3 ) of CytR or CRP were incubated in binding buffer ( 10 mM Tris HCl ( pH 7.8 ) , 50 mM KCl , 1 mM EDTA , 50 µg/ml acetylated bovine serum albumine , 1 mM dithiothreitol , 0.05 % Nonidet-P40 ) containing 20 µg/ml competitor DNA ( pGEM4 obtained from Promega ) for 30 min. at 37°C in a total volume of 10 µl ( 6 ) . |
| 2 | Briefly , slides were rehydrated in water , treated with 0.2 M HCl for 20 minutes at room temperature , rinsed with 10 mM RIS , 1 mM EDTA buffer , then treated with 20 µg/ml proteinase K in TRIS/EDTA buffer for 10 minutes . |
| 3 | At this distance , a 0·07 m length of Mg ribbon produces a coating of about 20 µm thickness . |
| 4 | HGT-1 cells were exposed sequentially to zero , 3 , 6.7 , 10 , and 20 µM 4-bromo-A23187 ( a non-fluorescent analogue of A23187 ) in NaCl solution containing 0.01% bovine serum albumin , which was included to prevent non-specific binding of ionophore to the chamber . |
| 5 | In the Survival Study , liquid vitamin A or placebo was supplied in opaque 150 mL bottles containing 20 IU/mL vitamin E alone ( placebo ) or plus 100,000 IU/mL retinol equivalent as retinyl palmitate ( vitamin A ) in purified peanut oil . |
| 6 | Net absorption of chloride was seen from solution I , which contained 140 mmol/l of chloride , but not from solution II , which contained only 20 mmol/l chloride . |
| 7 | DNA binding reactions were performed at room temperature in a 20 µl reaction mixture containing 100 mM NaCl , 20 mM N -2-hydroxyethylpiperazine- N' -ethanesulfonic acid ( HEPES ) -KOH ( pH 7.5 ) , 0.5 mM EDTA , 0.5 mM DTT , 0.025% Nonidet P-40 , 4% Ficoll , 5 ng/µl poly(dI-dC) , 100 ng/µl bovine serum albumin , 1–2 µl of 1:20 diluted fusion protein ( see above ) and 0.25 ng of labelled binding-site probe ( 10.000–20.000 cpm ) . |
| 8 | Something like the planned fleet of 20 B-2 Stealth machines may be enough , particularly if they are equipped with accurate conventional weapons . |
| 9 | ( Maintenance programme : Makes one 20 cm/8 inch cake , serving 8 ) |
| 10 | The new yarn is an 80 % Courtelle and 20 % wool , machine washable mix which will be popular in the current trend for Aran knits . |