Example sentences of "[vb pp] [prep] [num] [unc] [noun sg] " in BNC.
Next page| No | Sentence |
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| 1 | Briefly , the ethanolic extracts were dried under an air stream , mixed with 200 µl pyridine and acetic anhydride and kept for 18 hours in the dark , at room temperature . |
| 2 | Syrian golden hamsters were inoculated with 50000 H2T hamster ductal pancreatic cancer cells . |
| 3 | COS 7 cells ( 2 106 ) were transfected with 1 µg DNA and 500 µg DEAE-Dextran ( Pharmacia ) in 1 ml Hanks-balanced salt solution ( Kantonsapotheke Zürich ) for 30 min , shocked in 25% DMSO solution in Hanks for 3 min and then incubated for 30 hr in fresh DME-Medium containing10% FCS . |
| 4 | Portions of the supernatants were evaporated in a vacuum oven at 40°C , dissolved in 0.05 mol/l sodium acetate buffer ( pH 6.2 ) , and were analysed for cyclic AMP by using an acetylated [ 1 2 5 I ] radioimmunoassay procedure ( New England Nuclear kit ) . |
| 5 | For polymerase chain reaction assays , 5 µl supernatant sample was added to 45 µl reaction premix consisting of 67 mM Tris-HCl pH 8.8 , 16.6 mM NH 4 SO 4 , 3.35 mM MgCl 2 ( 6 mM for IS902-PCR ) , 1.7 mg/ml BSA , 0.2 mM of each of datp , dGTP , dCTP , dTTP , 300 ng each of primers P90 and P91 ( P102 and P103 for IS902-PCR ) , 10 mM β-mercaptoethanol , 10 mM Tris ; 0.1 mM EDTA and 2 units of Taq DNA polymerase ( Amplitaq T M Cetus ) . |
| 6 | For critical work , final lapping is done with 0.25 µm diamond paste . |
| 7 | This can be done with one trainee/one instructor or with one instructor in the role of parent and all trainees acting in consort . |
| 8 | Platelets were stirred in 400 µl buffer containing 2.6 mM KCl , 1 mM MgCl 2 , 137 mM NaCl , 12 mM NaHCO 3 , 1.3 mM CaCl 2 , 5.5 mM glucose , 0.25 gelatine , 1 mM creatine phosphate and 10 IU/ml creatine phosphokinase ( pH 7.4 ) . |
| 9 | This is so even though it had absolutely no contractual effect and operated to non-one 's detriment . |
| 10 | The data also show that immunohistochemical techniques were much better than histological analysis , despite measurements performed on 1 µm epon sections observed under oil-immersion opics . |
| 11 | Muscle strips were precontracted by 10 µM acetylcholine and the mean amplitude of steady state contractions was measured for two minutes before and after the addition of leucocytes . |
| 12 | Briefly , slides were rehydrated in water , treated with 0.2 M HCl for 20 minutes at room temperature , rinsed with 10 mM RIS , 1 mM EDTA buffer , then treated with 20 µg/ml proteinase K in TRIS/EDTA buffer for 10 minutes . |
| 13 | The single nuclei separated in this way were filtered through 37 µm nylon mesh ( Tokyo Screen , Tokyo ) and smeared on microscope slides using a Cytospin 3 ( Shandon Inc , England ) . |
| 14 | Median bicarbonate concentration in saliva determined by the former method was 28.1 mm/l ( 95% CI 19.79–38.74 ) compared with 14.74 mmol/l alkali in saliva determined by back titration ( 95% CI , 12.95–16.69 ) — that is , a 90% overestimation ( p= 0.003 ) . |
| 15 | Inspired by 70 's punk , this short feminine crop is by Nikki Kirk of Architect in Leeds |
| 16 | In Sudan , by contrast , forecasters claim to have visited all areas badly affected by 1990 's drought . |
| 17 | C41 films are developed in 2 min.40 sec. at 38 degrees C and bleach-fixed for 6 minutes — there is no rinse or stop bath between the two baths ( a special conditioner is supplied for Fuji films ) . |
| 18 | Muscle relaxation by cells was reduced by 200 nM oxyhaemoglobin and 10 µ M methylene blue but was increased by 60 n/ml superoxide dismutase . |
| 19 | HCO 3 - absorption was reduced by 6.25 µg cholera toxin and changed to secretion by the higher doses ( Table V ) . |
| 20 | HT-29 cells were cultured with 50 U/ml IFN γ with or without drug , and northern blot analysis was performed using a probe for the β chain of the DR molecule . |
| 21 | Serial sections were cut at 4–6 µm perpendicular to the mucosal surface . |
| 22 | Muscle relaxation by cells was reduced by 200 nM oxyhaemoglobin and 10 µ M methylene blue but was increased by 60 n/ml superoxide dismutase . |
| 23 | A geographer , Alfred Siemens of the University of British Columbia , has found rectilinear remains of canals and planting platforms ( raised fields ) in central Veracruz , which , he claims , are distinctly oriented about 1 5o east of north ( American Antiquity , vol 48. p 85 ) . |
| 24 | After washing and change of lap paper , polishing is continued with 1 µm diamond . |
| 25 | Cases reported here were associated with 50 µg oestrogen combined pills , mostly before this risk was recognised ; it remains to be seen whether 30 µg pills are safer , but as the progestogen component may carry a greater hypertensive risk than the oestrogen component all cases still need careful follow up at not less than three monthly intervals . |
| 26 | In some experiments , lysosomes were incubated with oleic acid which had been emulsified in 250 µM NaCl — 25 µM Tris buffer ( pH 7.4 ) by sonication under nitrogen as described by Nagai et al using a Heat Systems Cup Horn Sonicator at an output setting of four for 10 minutes at 25°C . |
| 27 | Net K + absorption was significantly decreased by 12.5 µgcholera toxin , and only the highest dose ( 25 µg ) caused net secretion ( Table IV ) . |
| 28 | The reaction mixtures were overlaid with 50 µl paraffin oil , then submitted to 33 cycles of IS900-PCR amplification or 40 cycles of IS902-PCR amplification . |
| 29 | Plasma gastrin concentration was determined in 200 µl plasma using a double antibody liquid phase 1 2 5 I-radioimmunoassay ( Diagnostic Products Corporation , Los Angeles , Calif , USA ) . |
| 30 | Created by Three 's Company 12 Flitcroft Street , London WC2 Produced by Angus Hudson Ltd , Mill Hill , London Typeset by Watermark , Cromer Printed in Great Britain |