Example sentences of "[verb] [prep] [num] [unc] [noun sg] " in BNC.
Next page| No | Sentence |
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| 1 | Briefly , the ethanolic extracts were dried under an air stream , mixed with 200 µl pyridine and acetic anhydride and kept for 18 hours in the dark , at room temperature . |
| 2 | Syrian golden hamsters were inoculated with 50000 H2T hamster ductal pancreatic cancer cells . |
| 3 | COS 7 cells ( 2 106 ) were transfected with 1 µg DNA and 500 µg DEAE-Dextran ( Pharmacia ) in 1 ml Hanks-balanced salt solution ( Kantonsapotheke Zürich ) for 30 min , shocked in 25% DMSO solution in Hanks for 3 min and then incubated for 30 hr in fresh DME-Medium containing10% FCS . |
| 4 | Portions of the supernatants were evaporated in a vacuum oven at 40°C , dissolved in 0.05 mol/l sodium acetate buffer ( pH 6.2 ) , and were analysed for cyclic AMP by using an acetylated [ 1 2 5 I ] radioimmunoassay procedure ( New England Nuclear kit ) . |
| 5 | For polymerase chain reaction assays , 5 µl supernatant sample was added to 45 µl reaction premix consisting of 67 mM Tris-HCl pH 8.8 , 16.6 mM NH 4 SO 4 , 3.35 mM MgCl 2 ( 6 mM for IS902-PCR ) , 1.7 mg/ml BSA , 0.2 mM of each of datp , dGTP , dCTP , dTTP , 300 ng each of primers P90 and P91 ( P102 and P103 for IS902-PCR ) , 10 mM β-mercaptoethanol , 10 mM Tris ; 0.1 mM EDTA and 2 units of Taq DNA polymerase ( Amplitaq T M Cetus ) . |
| 6 | L's utterance in line 45 begins with a comment on the photograph in London English ( " that 's me " ) but after a pause she switches to Creole to comment on V 's photography : " Valerie cut me off there , boy ! " which she follows with a laugh . |
| 7 | I can think of one c comment Chairman . |
| 8 | First , as between X and Y , Y is acting as X 's agent in selling to Z ; secondly , as between Y and Z , Y is selling as principal , Aluminium Industrie Vaasen BV v. Romalpa Aluminium Ltd. ( 1976 C.A. , for a fuller account see paragraph 3–26 below . ) |
| 9 | Much explanatory and archival material is on display and it will round off 1992 's anniversary celebrations : the discovery of the new World and the 125th year of the founding of the Canadian Confederation . |
| 10 | Breastfeeding does perky things to some women 's statistics , but it left mine looking like two well-past-their-sell-by-date helium balloons . |
| 11 | For critical work , final lapping is done with 0.25 µm diamond paste . |
| 12 | This can be done with one trainee/one instructor or with one instructor in the role of parent and all trainees acting in consort . |
| 13 | Incorporation of 0.7–1.1 nmol/l i ims 30 ( approximately 2 mci/mol ) into 6 nmol/l pure porcine VIP in 50 mmol/l acetate buffer at ph 5.0 was achieved by incubating with 22 pmol/l lactoperoxidase and 1.8 nmol/l H 2 O 2 at room temperature for 15 minutes in a total volume of 130 µl . |
| 14 | Granulocytes and mononculear cells produced concentration dependent relaxations of circular smooth muscle precontracted with 10 µM/l acetylcholine ( figs 1 and 2 ) . |
| 15 | The supernatant was transferred to a glass gas liquid chromatography vial ( Chromacol Ltd , London , UK ) dried and resuspended in 200 µl hexane . |
| 16 | The lipid mixture was evaporated under N 2 , lyophilised overnight and resuspended in 250 µM NaCl — 25 µM Tris buffer ( pH 7.4 ) . |
| 17 | ‘ Slade Alive ’ , what a brilliant album ! ’ gleams Gordon as he digs in early-'70s ' vinyl from behind a mound of Elvis ashtrays . |
| 18 | ‘ Slade Alive ’ , what a brilliant album ! ’ gleams Gordon as he digs in early-'70s ' vinyl from behind a mound of Elvis ashtrays . |
| 19 | The extract was dried under a stream of nitrogen , redissolved in 30 µl cloroform and spotted onto gel plates coated with silica ( Whatman HP-KF 10×10 cm ) . |
| 20 | Platelets were stirred in 400 µl buffer containing 2.6 mM KCl , 1 mM MgCl 2 , 137 mM NaCl , 12 mM NaHCO 3 , 1.3 mM CaCl 2 , 5.5 mM glucose , 0.25 gelatine , 1 mM creatine phosphate and 10 IU/ml creatine phosphokinase ( pH 7.4 ) . |
| 21 | This is so even though it had absolutely no contractual effect and operated to non-one 's detriment . |
| 22 | Sometimes X sells goods to Y who in turn sells them to Z. Sometimes , however , Y acts as X 's agent in selling to Z. In the former case Z buys his goods from Y and if they are not delivered or are defective Z can look to his seller , Y , and if necessary can sue him for breach of his contract of sale . |
| 23 | The data also show that immunohistochemical techniques were much better than histological analysis , despite measurements performed on 1 µm epon sections observed under oil-immersion opics . |
| 24 | Muscle strips were precontracted by 10 µM acetylcholine and the mean amplitude of steady state contractions was measured for two minutes before and after the addition of leucocytes . |
| 25 | It fits into two 6U VMEbus slots , enabling the Model 742i to outperform multiboard computers , which typically have more intensive development and support requirements , the company claims . |
| 26 | It fits into two 6U VMEbus slots , enabling the Model 742i to outperform multiboard computers , which typically have more intensive development and support requirements , the company claims . |
| 27 | Preincubation with staurosporine alone enhanced the cyclic AMP responses in HGT-1 cells incubated with 0.5 mmol/l histamine for five minutes at 20°C by 36 ( 7 ) % ( Table I ) . |
| 28 | Briefly , slides were rehydrated in water , treated with 0.2 M HCl for 20 minutes at room temperature , rinsed with 10 mM RIS , 1 mM EDTA buffer , then treated with 20 µg/ml proteinase K in TRIS/EDTA buffer for 10 minutes . |
| 29 | Analysis of the 40 childrens ' biopsy specimens showed that the intraepithelial lymphocyte count and the villous height/crypt depth ratio values allowed separation into groups ( with minimal overlap ) corresponding to ( a ) the prechallenge style ( group A and B together ) , ( b ) ostchallenge with 100 mg/ day of gliadin , and ( c ) postchallenge with 500 mg/day of gliadin . |
| 30 | The single nuclei separated in this way were filtered through 37 µm nylon mesh ( Tokyo Screen , Tokyo ) and smeared on microscope slides using a Cytospin 3 ( Shandon Inc , England ) . |