Example sentences of "[conj] [verb] for a further " in BNC.

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1 LPS-specific monoclonal antibody was added in a solution of PBS containing 0.05% Tween 20 , 1% BSA and 0.05% sodium azide ( PBSB-Tween ) and incubated for a further hour at room temperature .
2 After washing , 100 µl of anti-transferrin horseradish peroxidase conjugate in buffer was added at a dilution of 1 in 2000 and incubated for a further 60 minutes .
3 After washing , 100 µl of substrate ( tetramethylbenzidine in DMSO ( 10 mg/ml ) dilutes 1 in 100 in 0.15 M citrate buffer and containing H 2 O 2 to a final concentration of 1.3 M/l ) were added and incubated for a further 15 minutes .
4 Briefly , approximately 10 ng of purified protein A-fusion protein was preincubated in 20 µl of binding buffer ( 10 mM Hepes ( pH 7.9 ) , 60 mM KCl , 1 mM DTT , 1 mM EDTA , 0.25 mg/ml bovine serum albumin and 12% glycerol ) with appropriate competitor DNA for 5 min at room temperature , then 10 fmol ( approx. 20,000 cpm ) of 32 P-labelled , annealed octamer oligonucleotides were added and incubated for a further 20 min at room temperature .
5 After a 20 minute incubation at 22°C , MgCl 2 ( to 10mM ) , CaCl 2 ( to 2mM ) and 0.0625 units of DNase I were added , mixed and incubated for a further 60 seconds , after which time 20µg of proteinase K in 100µl of a buffer containing 1% SDS , 100µg/ml tRNA , 200mM NaCl , 20mM EDTA was added and the reaction incubated at 50°C for a further 20 mins .
6 Add the milk , bring to the boil and simmer for a further 10 minutes .
7 The pilot scheme will involve shops in five selected villages for the first year , and if successful will be extended and developed for a further three years .
8 Reduce the heat to gas mark 5/190&C/375&F and cook for a further 15–20min , until the quiches are set .
9 Add the sliced sausage to the pan and cook for a further 15min .
10 Add the vinegar and capers and cook for a further 2–3min .
11 Cover and cook over a medium heat for 5min , then add the carrots and celery and cook for a further 5min .
12 Bake for 10min , then lower the temperature to gas mark 4/180&C/350&F , brush with more butter and cook for a further 30–40min , until golden brown .
13 Bake for 15min , then reduce the heat to gas mark 5/190°C/375°F and cook for a further 10min , or until the pastry is browned and crisp .
14 Stir carefully and cook for a further 10 minutes .
15 Add other vegetables and seasoning and cook for a further 10 minutes .
16 Add all other ingredients except the olives and cook for a further 5 minutes .
17 Add the chopped mushrooms and cook for a further 5 minutes .
18 Add the mushrooms , sweetcorn and dill and cook for a further 5 minutes .
19 Add the mushrooms and pepper and cook for a further 10 minutes .
20 Add the beans and cook for a further 30 mins before serving .
21 Add the garlic , chilli and pepper and cook for a further 1 minute , stirring .
22 4 Add the walnuts and bourbon and cook for a further 5 min .
23 Remove the foil and cook for a further 50 to 10 min , until the cheese has melted and the trout is cooked .
24 Stir in the shallots and cook for about 2 minutes , then add the carrots and beans and cook for a further 2 minutes .
25 Add the courgettes and peppers , and cook for a further 3 minutes .
26 Cover and cook for a further 4 minutes .
27 Reduce heat to 170°C/325°F/Gas Mark 3 and cook for a further 35 minutes until the filling is set .
28 Add apple and cook for a further 2 minutes .
29 Add the skinned and chopped tomatoes and cook for a further 10 min .
30 Stir in the curry powder and cook for a further 2 min .
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