Example sentences of "[vb base] with [noun] [noun sg] " in BNC.
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| 1 | The Mam I- Sfa NI , Mam I- Sau 3A and Xba I- Pvu II deoP2 fragments were purified by electrophoresis on polyacrylamide gels after labelling of the 5'-end of the Mam I site with polynucleotide kinase in the presence of γ- 32 P-ATP and the 3'-end of the Xba I site with Klenow fragment in the presence of α- 32 P-dCTP. 0.8 nM of the labelled fragments and various concentrations ( indicated in the Legend in Fig. 3 ) of CytR or CRP were incubated in binding buffer ( 10 mM Tris HCl ( pH 7.8 ) , 50 mM KCl , 1 mM EDTA , 50 µg/ml acetylated bovine serum albumine , 1 mM dithiothreitol , 0.05 % Nonidet-P40 ) containing 20 µg/ml competitor DNA ( pGEM4 obtained from Promega ) for 30 min. at 37°C in a total volume of 10 µl ( 6 ) . |
| 2 | The probe is a 350 bp Rsa I- Cla I fragment comprising the entire deoP2 , 32 P-end-labelled at the 5'-end of the Cla I site with polynucleotide kinase and isolated from pJEL152 . |
| 3 | The Mam I- Sfa NI , Mam I- Sau 3A and Xba I- Pvu II deoP2 fragments were purified by electrophoresis on polyacrylamide gels after labelling of the 5'-end of the Mam I site with polynucleotide kinase in the presence of γ- 32 P-ATP and the 3'-end of the Xba I site with Klenow fragment in the presence of α- 32 P-dCTP. 0.8 nM of the labelled fragments and various concentrations ( indicated in the Legend in Fig. 3 ) of CytR or CRP were incubated in binding buffer ( 10 mM Tris HCl ( pH 7.8 ) , 50 mM KCl , 1 mM EDTA , 50 µg/ml acetylated bovine serum albumine , 1 mM dithiothreitol , 0.05 % Nonidet-P40 ) containing 20 µg/ml competitor DNA ( pGEM4 obtained from Promega ) for 30 min. at 37°C in a total volume of 10 µl ( 6 ) . |
| 4 | Chop onion and chicken into small pieces and cook with petits pois in water or , if desired , white wine with water , in the wok or frying pan for 10 minutes . |
| 5 | We acknowledge with gratitude permission to reprint these from The Principal , West Midlands College of Higher Education , and the Secretary , University of Birmingham . |
| 6 | Frances McAvennie , a former council roadsweeper from Glasgow 's Milton housing scheme , is not the kind of character you immediately associate with brasserie lifestyle . |
| 7 | Double glaze with bubble polythene to keep the warmth in during the coldest months . |
| 8 | It is likely that Tom Keating was painting with egg tempera ( made by combining egg yolk with dry pigment ) onto a varnish , probably a fine resin varnish such as mastic or , even more likely , varnish with linseed oil added , which was still wet . |
| 9 | Had they set some kind of booby-trap , say with CS gas ? |
| 10 | Various authors produce tables to show that their measures correlate with research performance or productivity in particular areas , but there is no agreement on a ‘ best-fit ’ measure ; they are used to measure different entities , such as national performance differences , output per unit of funding , subject differences , and so on ; and they are often of limited predictive value to policy makers . |
| 11 | The investigators are interested in the extent to which differentiation and focus strategies conflict with cost efficiency and how far new technology ( eg flexible , computer-aided manufacturing methods ) resolves this conflict . |
| 12 | The Survey describes the gneisses in this zone as : gneiss with cataclasite fabric meaning gneiss that has suffered crushing ; mashed gneiss meaning gneiss that has been severely crushed ; and pseudotachylite — close to the actual plane of the thrust , meaning gneiss that has been crushed so severely it is now a black , flinty , very fine grained or glassy rock . |
| 13 | It was in fact the first time she had thought of such a thing , and it did not seem to be a very good idea , but nothing irritated her more than being addressed in that Listen With Mother voice ( particularly as her daughter-in-law spoke to her children quite normally ) and it goaded her to contrariness . |
| 14 | Main picture : In the long , narrow living room , modern light fittings mix with period furniture |
| 15 | Brush the inside of the fish mould with vegetable oil or butter so that the chocolate does n't stick to it . |
| 16 | Several produce subtle chemical secretions which actually interfere with crop growth . |
| 17 | Place top layer of cake on filling , dredge with icing sugar and decorate with crystallised spring flowers . |
| 18 | Support composite thirteen , support motion two nine eight , we support with qualification motion two nine nine and support motion three O two . |
| 19 | Commercial cows sold to £950 for an Aberdeen Angus X heifer with heifer calf at foot for S Pinkerton , Crumlin and springing cows sold to £900 for an in-calf shorthorn X presented by S Watt , Deerfin . |
| 20 | Er the metal that forms two different chlorides solution react with sodium hydroxide solution to give precipitates , one coloured reddish brown the other green . |
| 21 | US officials publicly denied that the Bush administration intended to link the settlements issue with absorption aid . |
| 22 | Cut a tongue in each of the faces to be joined so that they interlock neatly , bind the joint with raffia and cover with grafting wax or bituminous tree paint . |
| 23 | Place the dough in a clean bowl and cover with cling film . |
| 24 | Alternatively , enclose in clean , clear polythene bags or cover with cling film to prevent moisture loss and stand them in a warm place . |
| 25 | Place in a shallow dish and cover with cling film until needed . |
| 26 | Smear this mixture all over the lamb , cover with cling film and leave the meat in a cool place to marinate overnight . |
| 27 | Smear this mixture all over the lamb , cover with cling film and leave the meat in a cool place to marinate overnight . |
| 28 | Or : ( for microwaving ) place the pears in a deep dish , e.g. soufflé type , pour wine sauce over them and cover with cling film . |
| 29 | Cover with cling film and refrigerate overnight . |
| 30 | Cover with cling film , and rest in a cool place for an hour . |