Example sentences of "[vb pp] with [adj] [unc] " in BNC.
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| 1 | Briefly , the ethanolic extracts were dried under an air stream , mixed with 200 µl pyridine and acetic anhydride and kept for 18 hours in the dark , at room temperature . |
| 2 | Briefly , the ethanolic extracts were dried under an air stream , mixed with 200 µl of pyridine and acetic anhydride and kept for 18 hours in the dark , at room temperature . |
| 3 | Duplex DNA , prepared and [ 32 P ] -5'-endlabelled as described above , was mixed with 10 µg Dcm or Dcm/Cys177Ser protein in 20 mM Tris-HCl , 1 mM EDTA , 10 mM β-mercaptoethanol , 50 mM NaCl , pH 7.5 in a total volume of 20 µl . |
| 4 | [ 32 P ] -5'-endlabelled or non-radioactive duplex DNA , prepared as described above , was mixed with 10 µg Dcm or Dcm/Cys177Ser protein in DCM reaction buffer . |
| 5 | Reactions were incubated at 30°C for 10 minutes after which they were mixed with 10 µl 4 SDS sample buffer , boiled for 5 minutes and resolved by SDS-PAGE . |
| 6 | In Piraeus the Pasok-Left Coalition candidate , Stelios Logothetis , won with 56.5 per cent , and the left also took Patras and Iraklion . |
| 7 | This time the ‘ do n't knows ’ won with 36 per cent ; Mr Brown , former California governor , took 33 per cent and Mr Clinton 30 per cent . |
| 8 | Syrian golden hamsters were inoculated with 50000 H2T hamster ductal pancreatic cancer cells . |
| 9 | Using a 110mm diameter piece of plastic tubing as a former , a pair of coils wound with 24 turns/coil of 0.2mm wire give a coil constant of 3.95 gauss/ampere . |
| 10 | That , admittedly self-selecting , sample of 1416 readers of the magazine voted with 67 per cent reckoning ESP a likely possibility . |
| 11 | Stylish 6.5 ’ alloy wheels are standard on the V6 version shod with 195/65 R15 tyres . |
| 12 | B : Inhibition of the binding of AR , RJ2.2.5 and Raji nuclear extracts to the X-box by anti-Fos antiserum ; binding reactions which did not contain anti-Fos ( — in lanes 3 , 6 and 9 ) were supplemented with 1 µg of non-immune antiserum . |
| 13 | During the four week gluten microchallenge the standard gluten free diet was supplemented with 100 mg/day of gliadin ( crude Gliadin , Sigma Chemical Company , St Louis , MO , USA ) in group A children and 500 mg/day of gliadin in group B. The daily gliadin was administered with some sugar in a single dose , 15 minutes before breakfast . |
| 14 | COS 7 cells ( 2 106 ) were transfected with 1 µg DNA and 500 µg DEAE-Dextran ( Pharmacia ) in 1 ml Hanks-balanced salt solution ( Kantonsapotheke Zürich ) for 30 min , shocked in 25% DMSO solution in Hanks for 3 min and then incubated for 30 hr in fresh DME-Medium containing10% FCS . |
| 15 | When the target cells were preincubated with 100 U/ml of IFN-γ for two days , the ADCC activity of the serum samples from six patients with ulcerative colitis was 23.9 ( 2.0 ) % . |
| 16 | For critical work , final lapping is done with 0.25 µm diamond paste . |
| 17 | As I have done with that erm |
| 18 | This can be done with one trainee/one instructor or with one instructor in the role of parent and all trainees acting in consort . |
| 19 | And the idea is that as we 've done with previous erm , discussions , if each one could come along armed with some information to do with healthy eating , erm , there 's a list on page a hundred and seventeen , that might start you off thinking . |
| 20 | Secured with most lap/diagonal seat belts . |
| 21 | By contrast , holoenzymes reconstituted with truncated α-subunits , show cooperative binding at the gal P1 promoter only . |
| 22 | the proliferation of technical manuals over the last twenty years has meant that the number of manuals supplied with complex aircraft/systems has increased to the point where it is practically impossible to access all the information in the ‘ library ’ ( Fig. 1 shows an example of technical manual growth rate within the Naval Air Systems Command ) ; |
| 23 | Some of my friends went to the City They 're better paid — but spare your pity ; There 's more to life than pounds and pence — My quality of life 's immense : A cosy office , nearly new , Equipped with latest PS2 . |
| 24 | The nuclei on the slides were stained with 1 µg/ml of 4',6-diamidino-2-phenylindole dihydrochloride ( DAPI ) ( Sigma , USA ) in 0.2 M TRIS chloride buffered saline for one hour at room temperature in the dark . |
| 25 | Transcription reactions were performed with 1 µg of linearized plasmid in the presence of 100 µCi of [ α-32P ] UTP ( Amersham , 800 µCi/mM ) , 2.5 mM unlabelled ribotriphosphates and 15 U of T7 RNA polymerase or SP6 polymerase ( Promega ) , in a final volume of 20 µl . |
| 26 | Southern analysis was performed with 30 µg of human genomic DNA on nylon membrane as described ( 36 ) . |
| 27 | Background painted with 3030 B70G , Collector , matt emulsion from the Definitions range by Dulux , £15.49/2.5 litres . |
| 28 | They 're linen painted with proper er ground pigments . |
| 29 | To confirm these results , whole RNA fractions extracted from both strains and treated with 100 µg of DNAse 1 ( to eliminate DNA contamination ) were directly hybridized ( slot-blot ) with srRNA or CO III mitochondrial probes and with the nuclear probe DNA 45S ( 21 ) ( not shown ) . |
| 30 | Crohn 's disease was treated with 50 mg/d azathioprine and 8 mg/d methylprednisone . |