Example sentences of "a [num] [unc] reaction " in BNC.
Next page| No | Sentence |
|---|---|
| 1 | In vitro kinase assays were performed in a 30 µl reaction which contained the following ; 10 µg HeLa nuclear extract ( 28 ) or 200 ng of purified DNA-PK ( a gift from C.Anderson ) , 250 ng substrate protein , 25 mM Hepes pH 7.5 , 12.5 mM MgCl 2 , 20% glycerol , 0.1% NP-40 , 100 mM KCl , 50 µM ATP ( containing 10 µCi [ γ 32 P ] ATP ; 6000 Ci/mmol ) and 1 mM DTT. 200 ng of linearised DNA template was added to the appropriate reaction as indicated in the text . |
| 2 | The final volume of the template depends on the volume of the PCR reaction e.g. PCR products from a 30 µl reaction are dissolved in 10 µl H 2 O and PCR products from a 50 µl reaction are dissolved in 30-50 µl H 2 O. |
| 3 | For specific amplification ( a and b ) , a 10 µl reaction mixture consisting of 1.0 ng of template , 1.5 mM MgCl 2 , 200 µM of each dNTP , 50 mM KCl , 10 mM Tris-HCl , pH 8.5 , 6.4 pmoles of each primer and 0.8 units of Taq DNA polymerase ( Cenbiot RS , Brazil ) was submitted to the following temperature profile : 95°C for 3 mins for denaturation followed by 25 cycles of 51°C for 1 min for annealing , 72°C for 3 mins for extension and 92°C for 1 min for denaturation . |
| 4 | Amplification was performed as described elsewhere but with a 25 µl reaction volume with 5 µl of sample added . |
| 5 | The final volume of the template depends on the volume of the PCR reaction e.g. PCR products from a 30 µl reaction are dissolved in 10 µl H 2 O and PCR products from a 50 µl reaction are dissolved in 30-50 µl H 2 O. |
| 6 | DNA binding reactions were performed at room temperature in a 20 µl reaction mixture containing 100 mM NaCl , 20 mM N -2-hydroxyethylpiperazine- N' -ethanesulfonic acid ( HEPES ) -KOH ( pH 7.5 ) , 0.5 mM EDTA , 0.5 mM DTT , 0.025% Nonidet P-40 , 4% Ficoll , 5 ng/µl poly(dI-dC) , 100 ng/µl bovine serum albumin , 1–2 µl of 1:20 diluted fusion protein ( see above ) and 0.25 ng of labelled binding-site probe ( 10.000–20.000 cpm ) . |