Example sentences of "then centrifuge at [num] [noun] " in BNC.
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| 1 | Cells were scraped into extraction buffer , incubated for 1h at 4°C with gentle vortexing every 10 min , and then centrifuged at 18,000 g for 20min . |
| 2 | This pooled concentrated urine was then centrifuged at 10000 g for five minutes and 1 ml of supernatant chromatographed on a Sephadex G-25 column ( 0.9×43 cm ) in TRIS buffered saline ( TBS ) containing 50 mM TRIS/HCl , 0.15 M NaCl , 3.1 mM NaN 3 at pH 7.3 at a flow rate of 39.5 ml/ hour and 1.056 ml fractions collected and assayed using the APGPR ELISA . |
| 3 | The suspensions were then centrifuged at 1500 rpm for 10 minutes . |
| 4 | The filtered solution was then centrifuged at 3 g for 5 minutes , the supernatant removed , and the cells resuspended in 10 ml of HBSS solution . |
| 5 | The resulting crude homogenate was then centrifuged at 600 g for 10 minutes to remove unbroken cells and nuclear debris . |