Example sentences of "in a [adj] µl [noun] " in BNC.
Next page| No | Sentence |
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| 1 | MKN45G xenografts were started in male nude mice ( Harlan-Olac , Bicester , UK ) ( weight 35 g , age 8–10 weeks ) by a subcutaneous injection of 10 7 cells ( in a 200 µl volume of sterile phosphate buffered saline ( PBS ) ) into the left hand flank . |
| 2 | 10 µl of this supernatant was used as template DNA in a 100 µl PCR reaction which included 2 units of Taq polymerase ( Cetus or Boehringer ) , reaction buffer with 2.5 mM MgCl 2 and dNTPs at a final concentration of 400 µM . |
| 3 | Histamine was given in doses of 1 , 5 , and 10 mgkg - 1 day - in a 100 µl injection directly into the site of the grafted tissue . |
| 4 | DNA binding reactions were performed at room temperature in a 20 µl reaction mixture containing 100 mM NaCl , 20 mM N -2-hydroxyethylpiperazine- N' -ethanesulfonic acid ( HEPES ) -KOH ( pH 7.5 ) , 0.5 mM EDTA , 0.5 mM DTT , 0.025% Nonidet P-40 , 4% Ficoll , 5 ng/µl poly(dI-dC) , 100 ng/µl bovine serum albumin , 1–2 µl of 1:20 diluted fusion protein ( see above ) and 0.25 ng of labelled binding-site probe ( 10.000–20.000 cpm ) . |
| 5 | In vitro kinase assays were performed in a 30 µl reaction which contained the following ; 10 µg HeLa nuclear extract ( 28 ) or 200 ng of purified DNA-PK ( a gift from C.Anderson ) , 250 ng substrate protein , 25 mM Hepes pH 7.5 , 12.5 mM MgCl 2 , 20% glycerol , 0.1% NP-40 , 100 mM KCl , 50 µM ATP ( containing 10 µCi [ γ 32 P ] ATP ; 6000 Ci/mmol ) and 1 mM DTT. 200 ng of linearised DNA template was added to the appropriate reaction as indicated in the text . |