Example sentences of "of 10 µl " in BNC.
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| 1 | Aliquots of 10 µl each were withdrawn from the mixture at various time points and quenched by adding 10 µl ice-cold 20% ( v/v ) aqueous trichloro acetic acid ( TCA ) . |
| 2 | NCp7 or NCp7 mutants were incubated with the indicated amount of DNA in a final volume of 10 µl in the buffer described above for 5 min at 37°C . |
| 3 | The Mam I- Sfa NI , Mam I- Sau 3A and Xba I- Pvu II deoP2 fragments were purified by electrophoresis on polyacrylamide gels after labelling of the 5'-end of the Mam I site with polynucleotide kinase in the presence of γ- 32 P-ATP and the 3'-end of the Xba I site with Klenow fragment in the presence of α- 32 P-dCTP. 0.8 nM of the labelled fragments and various concentrations ( indicated in the Legend in Fig. 3 ) of CytR or CRP were incubated in binding buffer ( 10 mM Tris HCl ( pH 7.8 ) , 50 mM KCl , 1 mM EDTA , 50 µg/ml acetylated bovine serum albumine , 1 mM dithiothreitol , 0.05 % Nonidet-P40 ) containing 20 µg/ml competitor DNA ( pGEM4 obtained from Promega ) for 30 min. at 37°C in a total volume of 10 µl ( 6 ) . |
| 4 | The RNA was resuspended in 5 µl of water and the primer was extended in a solution containing 50 mM Tris-HCl ( pH 7.5 ) , 40 mM KCl , 7 mM MgAc 2 , 2 mM DTT , 200 µM each dNTP ( except dATP which was 100 µM ) , 2 µCi of α ( 32 P ) dATP ( 400 Ci/mmol ) , 10 units of RNasin and 5 units of AMV-reverse transcriptase , in a total volume of 10 µl . |
| 5 | Two µl of the mixture ( corresponding to 0.6 µg of oligo ) were used in ligation reaction with 1 unit of T4 DNA ligase in a total volume of 10 µl ( 70 mM Tris-HCl ( pH 7.6 ) , 10 mM MgCl 2 and 5 mM dithiothreitol ) , plus 2.3 nM cold ATP . |