Example sentences of "[prep] [adj] minutes at " in BNC.
Next page| No | Sentence |
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| 1 | Prove for 1 hour , brush with beaten egg and bake for 30–40 minutes at 425°F ( 220°C ) Gas Mark 7 . |
| 2 | Mr Justice Roch said the court would start early at 10am and adjourn for 30 minutes at hourly intervals to allow the interpreters to rest and to ensure the defendant had understood the evidence . |
| 3 | For if you are not exercising aerobically using some form of whole body continuous movement ( brisk walking , jogging , cycling ) for 30 minutes at least three to four times a week , then by definition , you are sedentary . |
| 4 | Transfer the mixture to an ovenware glass dish and heat in an oven for 30 minutes at 150 -C ( 300 -F ) to sterilize it . |
| 5 | The membrane was blocked for 30 minutes at 20°C in PBS containing 5% non-fat dried milk powder . |
| 6 | After incubation for 30 minutes at 42°C , the reaction was chilled to 4°C and 10 µl of 0.5 KGB buffer plus 10 units of E.coli DNA polymerase 1 and 0.4 units of RNaseH was added . |
| 7 | The International Atomic Energy Agency requires flasks used for transporting spent fuel to be designed to survive the impact of a 9-m fall — equivalent to an impact of 30 mph — and to survive being engulfed by fire for 30 minutes at a temperature of 800 deg . |
| 8 | After washing in PBS , streptavidin-peroxidase ( jackson Immunonuclear Laboratories , Westgrove , PA , USA ) was applied to the sections at a 1:5000 dilution in PBS with 1% BSA for 30 minutes at room temperature . |
| 9 | The slides were washed in 0.5 M NaCl , 10 mM TRIS pH 7.4 , 1 mM EDTA for 15 minutes , then digested with RNAse A at 20 µg/ml in the same buffer for 30 minutes at 37°C . |
| 10 | After incubation for 30 minutes at 37°C , samples were centrifuged for a minite at 10000 g . |
| 11 | Culture medium was removed , and the cell layers washed with a buffered NaCl solution containing ( mM ) : Na + , 140 ; K + , 4.5 ; Cl - , 149 ; Ca 2 + , 1.2 ; Mg 2 + , 1.2 ; glucose 10 ; and 4- ( 2-hydroxyethyl ; -1-piperazine ethanesulphonic acid ( HEPES ) 10 , titrated to pH 7.4 with NaOH , and incubated with [ 3 H ] omeprazole ( 1.25 nmol/ well ; equivalent to about 3×10 5 counts per min ) in the presence or absence of 1 mM histamine for 30 minutes at 37°C . |
| 12 | The mixtures were incubated for 30 minutes at 25°C and centrifuged for 10 minutes . |
| 13 | The solution was centrifuged at 1700 g for 30 minutes at 4°C . |
| 14 | Filters were washed twice in 2×SSC ( 1×SSC is 0.15 M NaCl plus 0.015 M sodium citrate ) containing 0.5% SDS for 30 minutes at room temperature and then in 0.1×SSC containing 0.1% SDS at 50°C for 30 minutes before autoradiography . |
| 15 | Endogenous peroxidase activity was blocked with 0.3% hydrogen peroxide in 0.1% sodium azide for 30 minutes at room temperature . |
| 16 | After rinsing in PBS , acid hydrolysis was performed by incubating the sections in 2 N HCl for 30 minutes at room temperature followed by washing in two successive baths ( three minutes each ) of 0.1 M borax ( Na 2 B 4 O 7 ) at pH 8.5 to neutralise the acid . |
| 17 | In the immunocytochemical staining procedure , sections are digested gently by incubation in 0.02% Saponin for 30 minutes at room temperature ( Wargovich , personal communication ) . |
| 18 | Mucosal strips were incubated in Ca + + /Mg + + free Krebs-Henseleit saline , pH 7.4 , with 10 mM EDTA and 20 mM dithiothreitol , for 30 minutes at 37°C . |
| 19 | Pancreatic lysosomes from chow fed rats were incubated for 30 minutes at 37°C with ethanol , acetaldehyde or phosphatidylcholine vesicles containing cholesteryl oleate . |
| 20 | Isolated pancreatic lysosomes were incubated with ethanol or acetaldehyde in tightly sealed tubes for 30 minutes at 37°C . |
| 21 | Isolated pancreatic lysosomes were incubated with phosphatidylcholine vesicles containing cholesteryl oleate for 30 minutes at 37°C . |
| 22 | After washing and filtration , the nuclei were stained in propidium iodide solution ( 50 µg/ml ) containing RNAase ( 1 mg/ml ) for 30 minutes at 4°C and analysed in an EPICS CS flowcytometer ( Coulter Corporation ) . |
| 23 | After washing with buffer ( phosphate buffered saline , PBS , with 0.05% Tween 20 ) , serum samples ( diluted 1:10 in PBS-Tween ) were applied for 30 minutes at room temperature . |
| 24 | Intact crypts were isolated by incubating fresh lesion free colorectal mucosa in calcium and magnesium free Hanks ' balanced salt solution ( CMFH ) containing 30 mM EDTA for 30 minutes at 37°C and then agitating in CMFH . |
| 25 | After incubating for 30 minutes at room temperature , the reaction was stopped by washing the sections in cold water . |
| 26 | After removal of unbound primary antibody , the sections were incubated with monoclonal mouse anti-rabbit immunoglobulin ( 1/100 , Dako ) for 30 minutes at room temperature before the application of rabbit anti-mouse immunoglobulin and APAAP complex . |
| 27 | After forty minutes at his ceiling of fifteen thousand feet , Killion felt drowsy and slow , like his aeroplane . |
| 28 | After ten minutes at RT , beads were captured using a magnetic rack and five stringent washes with 0.1 SSC were done . |
| 29 | OMAGH Town 's inability to get the ball into the net during half an hour of incessant first half pressure was cruelly punished by the deadly Billy Totten after 33 minutes at St. Julians Road this afternoon . |
| 30 | Pompey caught their Italian visitors cold after 16 minutes at chilly Fratton Park with Paul Walsh 's second goal of the season . |