Example sentences of "[verb] at [num] g " in BNC.
Next page| No | Sentence |
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| 1 | Homogenates were centrigued at 40000 g for 90 minutes at 4°C , and the pellet was resuspended in homogenisation buffer . |
| 2 | ( v ) Resuspend the pellet in 5 m1 of 45% glacial acetic acid , then immediately centrifuge at 150 g for 5 min . |
| 3 | Draw off the cell suspension and centrifuge at 150 g for 10 min in a conical centrifuge tube . |
| 4 | Centrifuge at 50 g for 5 min with slow acceleration . |
| 5 | Leyland says a 16-tonne vehicle braking at 0.5 g from 50 km/h dumps more than 1000 kW into the brakes as heat . |
| 6 | The free and protein bound leucine in the tissue biopsies were separated by pulverising the frozen tissue , homogenising in 0.2 M HClO 4 at 4°C and centrifuging at 2800 g for 20 minutes . |
| 7 | The samples were immediately ultracentrifuged at 100000 g for two hours at 37°C ( Hitachi 55P-72 , Tokyo , Japan ) . |
| 8 | The resultant supernatant was removed immediately and respun at 300 g to yield a cell free plasma . |
| 9 | The supernatant was removed immediately and respun at 300 g for 10 minutes to yield cell free plasma . |
| 10 | The thawed samples were spun at 1000 g for 10 minutes and 500 µl aliquots were taken into a second acid washed plastic tube , diluted 1:9 with ultrapure 0.11 M nitric acid , and analysed by ICPES ( Philips PV 8050 ) at the following wavelengths : potassium 766.49 nm , sodium 588.99 nm , magnesium 383.83 nm , copper 324.75 nm , calcium 315.89 nm , iron 259.94 nm , and zinc 213.86 nm . |
| 11 | Samples were thawed , vortexed , and then centrigudes at 10000 g for one minute . |
| 12 | Phytoplankton of diatoms and other green algae is poorly developed ; chlorophyll a values of up to 8.0 µg 1 -1 were recorded , and annual production was estimated at 12.6 g C m -2 in a year ( Ellis-Evans , 198 la ) . |
| 13 | ( iii ) Centrifuge the cell suspension obtained at 50 g for 5 min . |
| 14 | For this the samples were thawed and centrifuged at 3000 g for 10 minutes to remove the mucus . |
| 15 | Each blood sample was immediately made 20 mM with zinc acetate , allowed to clot , and within 2.5 hours of collection , centrifuged at 3000 g for 20 minutes , the separated serum mixed with an equal volume of ELISA immunoassay buffer containing 50 mM TRIS/HCL , 0.05% ( wt/vol ) casein , 3.1 mM NaN 3 , 10 mM ethylenediaminetetra-acetic acid , 0.05% ( wt/vol ) Tween 20 at pH 7.2–7.4 , and suspended in a boiling bath for 10 minutes . |
| 16 | The supernatant was removed and centrifuged at 100 g for five minutes . |
| 17 | Five millilitres of cell free plasma were added to the cell suspension and centrifuged at 100 g for five minutes . |
| 18 | The supernatant was removed and centrifuged at 100 g for 5 minutes . |
| 19 | Five ml of cell free plasma were then added to the cell suspension and centrifuged at 100 g for 5 minutes . |
| 20 | After reflux the sample was cooled on ice and centrifuged at 1500 g for 15 minutes . |
| 21 | After removal of the stomach , a 50–100 mg full thickness strip from the oxyntic region was immediately homogenised in 3.5 ml of 0.01 M hydrochloric acid and centrifuged at 2000 g for 15 minutes before removal of the supernatant for the following measurements : |
| 22 | Cells were scraped into extraction buffer , incubated for 1h at 4°C with gentle vortexing every 10 min , and then centrifuged at 18,000 g for 20min . |
| 23 | Supernatants were combined and centrifuged at 16500 g for 25 minutes to obtain a lysosome enriched pellet . |
| 24 | Leucocytes were washed twice in 10 ml solution ( 37°C ) containing 100 nM/l indomethacin ( KHI ) to inhibit prostglandin synthesis , centrifuged at 200 g for 10 minutes , and resuspended in 5 ml KHI . |
| 25 | One portion of the homogenates was boiled for 10 minutes , centrifuged at 5000 g for 10 minutes and the supernatant stored at -20°C for the later determination of histamine . |
| 26 | Blook ( 5 ml ) was layered above an 8 ml bilayer of hypaque 1017 and 1119 in equal volumes and centrifuged at 700 g for 25 minutes . |
| 27 | Briefly , tumours or mucosal scrapings were homogenised in TEA buffer ( 250 mM sucrose , 10 mM triethanolamine ( pH 7.6 ) containing 1 mM phenylmethylsuphonylfluoride ( PMSF ) and 1 mM N-ethylmaleimide as protease inhibitors ) on ice and centrifuged at 1000 g for 10 minutes . |
| 28 | Cell ( 10 6 ) were added to 5ml Falcon polystyrene tubes ( Becton Dickinson , Lincoln Park , NJ , USA ) , and centrifuged at 1000 g for 5 minutes . |
| 29 | Serum samples were stored at -80°C without preservatives and centrifuged at 105000 g for 90 minutes before use . |
| 30 | Oral acyclovir has been maintained at 4 g orally per day . |