Example sentences of "[verb] [adv] as described " in BNC.
Next page| No | Sentence |
|---|---|
| 1 | Isolation of total RNA , purification of poly ( A ) + RNA from zebrafish embryos at different developmental stages , gel electrophoresis and Northern blot analysis were carried out as described previously ( 54 ) . |
| 2 | Isolation of nucleic acids , gel electrophoresis , hybridization conditions , oligonucleotide synthesis and standard in vitro recombinant techniques were carried out as described elsewhere ( 27 , 28 ) . |
| 3 | The expression and purification of the two subunits of yTFIIA was carried out as described ( 17 ) and they were assayed by 17.5% SDS-PAGE and subsequent staining with coomassie blue . |
| 4 | Assays of CAT activity were carried out as described by Gorman ( 19 ) with extracts which were equalised for protein content , as determined by the method of Bradford ( 20 ) . |
| 5 | Endothelin induced gastric mucosal damage was carried out as described below . |
| 6 | Similar procedures were carried out as described above except that the secondary antibody was rabbit antibody to human immunoglobulin conjugated with peroxidase ( DAKO , High Wycombe , UK ) instead of alkaline phosphatase . |
| 7 | Exactly the same procedure was carried out as described above . |
| 8 | Filter hybridisation was carried out as described by Southern . |
| 9 | Immunoprecipitation with mABs was carried out as described by Sonnenberg et al with the modification : cell extracts were first incubated overnight at 4°C with 10 µl of the specific antibody and 10 µl of a species specific polyclonal rabbit lgG ( Dako , Hamburg , Germany ) antibody and then for an incubation period of one hour with 9 mg protein A sepharose at 4°C . |
| 10 | DNase I sensitivity assays were carried out as described by Enver et al. |
| 11 | Hybridisation and S1 protection reactions were carried out as described previously ( 16,17 , also see below ) . |
| 12 | This can be done electrically as described above . |
| 13 | The module can then be scanned again as described above . |
| 14 | Nuclear extracts from Hep3B cells ( either untreated or stimulated with 1000U/ml IL-6 ) were prepared essentially as described ( 34 ) except that the buffers contained the phosphatase inhibitors , sodium fluoride ( 50mM ) and sodium pyrophosphate ( 10mM ) . |
| 15 | Peptide stimulations and CTL assays were done essentially as described in the legend to Fig. 2 . |
| 16 | Th IN cleavage reaction was performed basically as described previously ( 22 ) . |
| 17 | The EMSA and the DNA probes used were performed exactly as described in [ 14 ] . |
| 18 | The transfection was performed exactly as described for stationary cells except all procedures were carried out with medium containing 10% serum . |
| 19 | DNA isolation , restriction enzyme digestion , agarose gel electrophoresis , Southern blot transfer , and hybridization were performed essentially as described ( 36 ) . |
| 20 | Tissue type plasminogen activator antigen was measured essentially as described by Rijken et al . |
| 21 | IgG anti-bovine lactoferrin antibodies were measured essentially as described above , except that no negative or positive serum references were used . |
| 22 | This file should contain the responses the user would have given to the prompts for the relevant options had the system been used interactively as described below . |
| 23 | The amount in quadruplicate of 3 H-thymidine incorporated was assessed biochemically as described by Selden and Hodgson . |
| 24 | However , it would certainly confuse the fox , thereby causing it to run erratically as described . |
| 25 | When the Module Version Management facilities are required , the configuration file should be set up as described below . |
| 26 | liability whilst travelling abroad as described above , provided notification of the trip has been given to us |
| 27 | Neither is there any sign that epithelial cells further from the leading edge are putting out lamellipodia or crawling forward as described in some adult systems . |
| 28 | I have enjoyed the co-operation of Biblical Fundamentalists ( who do not accept the theory of evolution , but believe that the world was created exactly as described in Genesis I ) . |
| 29 | Release of prostaglandin E2 , 6k prostaglandin F1a , and thromboxane B2 into the incubation media was determined radioimmunologically as described previously . |
| 30 | Flow cytometric analysis of expression of integrins on inhibitor treated and untreated HT-29 cells was conducted essentially as described . |