Example sentences of "then [vb past] with " in BNC.
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| 1 | Normal human neutrophils were cytocentrifuged , ethanol fixed , and then incubated with coded patients ' sera . |
| 2 | After blocking free protein binding sites with 0.5% Tween 20 ( Sigma ) for 2 hours , the filter was incubated with patient 's sera ( diluted 1:10 in TBS with 0.5% Tween 20 ) for 30 minutes , washed in 0.5% Tween in TBS , and then incubated with the rabbit anti-human immunoglobulin conjugated with alkaline phosphatase for a further 30 minutes . |
| 3 | Preparations were incubated with the primary antibody for 60 minutes , rinsed in Tris buffered saline and then incubated with the secondary antibody ( mouse anti-rabbit , diluted in Tris buffered saline/normal human serum ) for 30 minutes . |
| 4 | The sections were incubated in 5% normal rabbit serum to reduce background staining and then incubated with the monoclonal antibody to bromodeoxyuridine ( Dakopatts , Denmark ) diluted 1:100 with phosphate buffer for one hour . |
| 5 | The wood of the planks was almost white , but it had been sanded and then varnished with gums and resins until it glistened . |
| 6 | No wonder he then passed with very high marks . |
| 7 | It was some days before he resolved on this , but he then met with strong opposition from both Joffre and Foch , who wanted him first to penetrate the centre ridge between Pozières and Thiepval . |
| 8 | As he raised her hand towards his mouth his grey eyes widened , then gleamed with laughter as he saw the state it was in . |
| 9 | ‘ We had builders here for six months before we moved in , and then lived with them for another year , ’ says Sally , who was not only busy with their two young daughters , but was also running a full-time interior decoration business in Cirencester . |
| 10 | And er I 've been there all my life and that 's and that 'll be eighty seven years old come May and er I 've just worked on the farm all my days and er then of course when my people died I just er stayed myself , and then lived with other people here and there , just to help them . |
| 11 | He later confessed to having taken part in the theft of a quantity of goods including hi-fi equipment , a television , a leather jacket and a carriage clock , totalling value of £1,222 , from the bedsit next door to the one which he then shared with a fridge at Spitalhatch , Alton . |
| 12 | Five minutes had now passed since the capture , and Ulysse left the site with the upper half of the monkey , which he then shared with Brutus . |
| 13 | The poles slid glistening in and out of the water as the craft approached and then beached with a bump . |
| 14 | The village of Shiptonthorpe lies a short distance west of Market Weighton on the main York to Hull road Until the year 1876 it was known as Shipton , it then amalgamated with Thorpe Le Street . |
| 15 | She was still breathing evenly upon both bottle and net , when suddenly the net expanded and then deflated with an abrupt whooshing sound . |
| 16 | More than one was caught by a pair of squealing girls , who gleefully anointed them with kitchen grease and then fled with the warrior clanking in pursuit . |
| 17 | The protein precipitate was washed with HClO 4 , dissolved in 0.3 M NaOH at 37°C for one hour and then reprecipitated with 2 M HClO 4 . |
| 18 | Or they might be mixed with onion and garlic softened in butter then stewed with carrots and rosemary to go with roast lamb for lunch . |
| 19 | But then avowed with a great deal of feeling , ‘ I sorely needed somebody 's help when , with daylight I returned to that hotel and discovered you 'd taken a train back to Mariánské Láznë ! ’ |
| 20 | England then equalised with a controversial Webb penalty seven minutes before the break to leave it 3–3 at the interval . |
| 21 | Ally McCoist missed an early sitter and then equalised with a beautifully flighted chip after Mark Hateley had set things up . |
| 22 | T'zin picked up the cup , then gestured with it . |
| 23 | Robyn asked , just a little shakily as her mind raced ahead to the possibilities before her and then retreated with equal speed , because her mind did n't like what it saw . |
| 24 | He dropped the bag over and then climbed with the blanket and the torch . |
| 25 | The rebels held their fire till the dragoons were within ten yards [ 9 m ] of them , then replied with ‘ a very sharp , popping kind ’ of volley which did such execution that one bystander ‘ saw daylight through them in several places ’ . |
| 26 | He gazed at her in silence for several seconds , then replied with icy simplicity , ‘ No time . |
| 27 | It was only after the women had left that she thought to check the receipt book to make sure it had not been her duty to collect payment for today 's four lunches , then sighed with relief when she saw that this had been attended to at lunchtime by Jean . |
| 28 | Theresa smiled and then nodded with serious satisfaction in a moment of grave feminine conspiracy . |
| 29 | He chewed away for a few moments and then nodded with pleasure . |
| 30 | His wife packed a box with various goodies for Leonora to take to Penry , then walked with them to the jetty , and waved them off on the Sea-Fret , a sturdy fishing-boat a lot newer than Penry 's . |