Example sentences of "and [vb past] [prep] a far [adj] " in BNC.
Next page| No | Sentence |
|---|---|
| 1 | After washing , 100 µl of anti-transferrin horseradish peroxidase conjugate in buffer was added at a dilution of 1 in 2000 and incubated for a further 60 minutes . |
| 2 | After a 20 minute incubation at 22°C , MgCl 2 ( to 10mM ) , CaCl 2 ( to 2mM ) and 0.0625 units of DNase I were added , mixed and incubated for a further 60 seconds , after which time 20µg of proteinase K in 100µl of a buffer containing 1% SDS , 100µg/ml tRNA , 200mM NaCl , 20mM EDTA was added and the reaction incubated at 50°C for a further 20 mins . |
| 3 | Briefly , approximately 10 ng of purified protein A-fusion protein was preincubated in 20 µl of binding buffer ( 10 mM Hepes ( pH 7.9 ) , 60 mM KCl , 1 mM DTT , 1 mM EDTA , 0.25 mg/ml bovine serum albumin and 12% glycerol ) with appropriate competitor DNA for 5 min at room temperature , then 10 fmol ( approx. 20,000 cpm ) of 32 P-labelled , annealed octamer oligonucleotides were added and incubated for a further 20 min at room temperature . |
| 4 | After washing , 100 µl of substrate ( tetramethylbenzidine in DMSO ( 10 mg/ml ) dilutes 1 in 100 in 0.15 M citrate buffer and containing H 2 O 2 to a final concentration of 1.3 M/l ) were added and incubated for a further 15 minutes . |
| 5 | After three hours incubation at 37°C the medium was replaced with fresh medium and reincubated for a further three hours . |
| 6 | The faecal pellet was resuspended in 45 ml 80% ethanol and refluxed for a further two hours . |
| 7 | The faecal pellet was then resuspended in 45 ml chloroform-methanol ( 1:1 ; vol:vol ) and refluxed for a further one hour . |