Example sentences of "and [verb] for a far [adj] " in BNC.
Next page| No | Sentence |
|---|---|
| 1 | After washing , 100 µl of anti-transferrin horseradish peroxidase conjugate in buffer was added at a dilution of 1 in 2000 and incubated for a further 60 minutes . |
| 2 | After a 20 minute incubation at 22°C , MgCl 2 ( to 10mM ) , CaCl 2 ( to 2mM ) and 0.0625 units of DNase I were added , mixed and incubated for a further 60 seconds , after which time 20µg of proteinase K in 100µl of a buffer containing 1% SDS , 100µg/ml tRNA , 200mM NaCl , 20mM EDTA was added and the reaction incubated at 50°C for a further 20 mins . |
| 3 | Briefly , approximately 10 ng of purified protein A-fusion protein was preincubated in 20 µl of binding buffer ( 10 mM Hepes ( pH 7.9 ) , 60 mM KCl , 1 mM DTT , 1 mM EDTA , 0.25 mg/ml bovine serum albumin and 12% glycerol ) with appropriate competitor DNA for 5 min at room temperature , then 10 fmol ( approx. 20,000 cpm ) of 32 P-labelled , annealed octamer oligonucleotides were added and incubated for a further 20 min at room temperature . |
| 4 | After washing , 100 µl of substrate ( tetramethylbenzidine in DMSO ( 10 mg/ml ) dilutes 1 in 100 in 0.15 M citrate buffer and containing H 2 O 2 to a final concentration of 1.3 M/l ) were added and incubated for a further 15 minutes . |
| 5 | Add the milk , bring to the boil and simmer for a further 10 minutes . |
| 6 | The pilot scheme will involve shops in five selected villages for the first year , and if successful will be extended and developed for a further three years . |
| 7 | Cover and cook for a further 4 minutes . |
| 8 | Add all other ingredients except the olives and cook for a further 5 minutes . |
| 9 | Add the chopped mushrooms and cook for a further 5 minutes . |
| 10 | Add the mushrooms , sweetcorn and dill and cook for a further 5 minutes . |
| 11 | 4 Add the walnuts and bourbon and cook for a further 5 min . |
| 12 | Add the drained vegetables and cook for a further 5 minutes , stirring all the time . |
| 13 | When the meat is very tender , add the mushrooms and cook for a further 5 minutes . |
| 14 | Add the cauliflower and courgettes and cook for a further 5 minutes . |
| 15 | Stir in tomato puree and fresh tomatoes and cook for a further 5 minutes , or until sauce is mushy and thickened . |
| 16 | cook for about 5 minutes each side ; then reduce heat and cook for a further 5 minutes each side . |
| 17 | Add the garlic , chilli and pepper and cook for a further 1 minute , stirring . |
| 18 | Stir carefully and cook for a further 10 minutes . |
| 19 | Add other vegetables and seasoning and cook for a further 10 minutes . |
| 20 | Add the mushrooms and pepper and cook for a further 10 minutes . |
| 21 | Add the skinned and chopped tomatoes and cook for a further 10 min . |
| 22 | Add red lentils and cook for a further 10 minutes until they are tender but still retain their shape . |
| 23 | Reduce heat to 170°C/325°F/Gas Mark 3 and cook for a further 35 minutes until the filling is set . |
| 24 | Add the courgettes and peppers , and cook for a further 3 minutes . |
| 25 | Add the mussels to the soup and cook for a further 3 minutes . |
| 26 | Add the beans and cook for a further 30 mins before serving . |
| 27 | Stir in the shallots and cook for about 2 minutes , then add the carrots and beans and cook for a further 2 minutes . |
| 28 | Add apple and cook for a further 2 minutes . |
| 29 | Stir in the curry powder and cook for a further 2 min . |
| 30 | Then reduce the oven temperature to 190°C , 375°F , Gas 5 and cook for a further 20 to 15 min . |